The role of polymerase chain reaction and its newer developments in feline medicine
Identifieur interne : 001168 ( Main/Exploration ); précédent : 001167; suivant : 001169The role of polymerase chain reaction and its newer developments in feline medicine
Auteurs : H. Lutz [Suisse] ; C. Leutenegger [Suisse] ; R. Hofmann-Lehmann [Suisse]Source :
- Journal of Feline Medicine and Surgery [ 1098-612X ] ; 1999.
English descriptors
- Teeft :
- American journal, Amplification, Analytical specificity, Assay, Assay conditions, Base pairs, Blood samples, Capsid protein gene, Cat, Cell culture, Chain reaction, Citrate synthase gene, Clinical microbiology, Clinical signs, Conjunctival swabs, Conjunctivitis, Constant amounts, Cornea1 sequestration, Diagnostic specificity, Domestic cats, Eosinophilic keratitis, Equivalence point, Ethidium bromide, Exogenous felv, Fcov, Fcov infection, Fehr, Feline, Feline calicivirus, Feline coronavirus, Feline coronaviruses, Feline genes, Feline herpesvirus, Feline immunodeficiency virus, Feline leukemia virus, Feline leukemia virus detection, Feline medicine, Felv, Felv infection, Felv proviral, Felv provirus, Field conditions, Fluorescence signal, Gene, Gene bank, Good correlation, Healthy animals, Healthy cats, High proviral load, High sensitivity, Hypervariable region, Immunodeficiency, Infectious diseases, Infectious virus, Input target, Leutenegger, Ligase chain reaction, Lutz, Microbiology, Mullis faloona, Newer assays, Normal cats, Nuclease activity, Pathogenesis, Peripheral blood, Pistello, Polymerase, Polymerase chain reaction, Positive test, Positive tests, Primer, Proviral, Proviral load, Provirus, Puma lentiviruses, Quantitation, Recent study, Respiratory tract disease, Retrovirus research symposium, Sequence analysis, Sick cats, Swab, Taqman, Taqman assay, Taqman method, Taqman probe, Taqman procedure, Taqman technology, Ultraviolet light, Vaccine, Veterinary immunology, Veterinary microbiology, Veterinary research, Viral, Viral load, Virological methods, Virology, Whole sequence.
Abstract
Abstract: We give a brief overview on the principles of the polymerase chain reaction (PCR), reverse transcriptase PCR (RT-PCR), quantitative competitive PCR and real-time PCR (TagMan technology). The literature dealing with PCR and its role in the diagnosis, pathogenesis and research of infectious diseases of the domestic cat is reviewed. Cross-contaminations which occasionally occur during handling of amplified DNA may be an important problem in the PCR laboratory. In many infectious diseases, PCR results are difficult to interpret as their predictive positive and negative values are not always known. Newer assays, such as TagMan procedures, are becoming increasingly reliable and cost-effective. It can be expected that additional knowledge on how to interpret PCR results will soon be available.
Url:
DOI: 10.1016/S1098-612X(99)90065-7
Affiliations:
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The literature dealing with PCR and its role in the diagnosis, pathogenesis and research of infectious diseases of the domestic cat is reviewed. Cross-contaminations which occasionally occur during handling of amplified DNA may be an important problem in the PCR laboratory. In many infectious diseases, PCR results are difficult to interpret as their predictive positive and negative values are not always known. Newer assays, such as TagMan procedures, are becoming increasingly reliable and cost-effective. It can be expected that additional knowledge on how to interpret PCR results will soon be available.</div></front></TEI><affiliations><list><country><li>Suisse</li></country><region><li>Canton de Zurich</li></region><settlement><li>Zurich</li></settlement><orgName><li>Université de Zurich</li></orgName></list><tree><country name="Suisse"><region name="Canton de Zurich"><name sortKey="Lutz, H" sort="Lutz, H" uniqKey="Lutz H" first="H." last="Lutz">H. Lutz</name></region><name sortKey="Hofmann Lehmann, R" sort="Hofmann Lehmann, R" uniqKey="Hofmann Lehmann R" first="R." last="Hofmann-Lehmann">R. Hofmann-Lehmann</name><name sortKey="Leutenegger, C" sort="Leutenegger, C" uniqKey="Leutenegger C" first="C." last="Leutenegger">C. Leutenegger</name><name sortKey="Lutz, H" sort="Lutz, H" uniqKey="Lutz H" first="H." last="Lutz">H. Lutz</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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